Anticorps Polyclonal de lapin anti-LIFR

• Phare
• Validé par KD/KO

LIFR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 22779-1-AP

Synonymes

CD118, LIF R, LIF receptor, LIFR, SJS2, STWS, SWS

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 22779-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22779-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 22779-1-AP

  IP Result of anti-LIFR (IP:22779-1-AP, 4ug; Detection:22779-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

• 

  IHC staining of human skeletal muscle using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse cerebellum using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 22779-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 22779-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22779-1-AP (LIFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 22779-1-AP

  1X10^6 HeLa cells were stained with .2ug LIFR antibody (22779-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu de cervelet de souris, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

22779-1-AP cible LIFR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIFR Protéine recombinante Ag18742
• Nom complet: leukemia inhibitory factor receptor alpha
• Masse moléculaire calculée: 1097 aa, 124 kDa
• Poids moléculaire observé: 190 kDa
• Numéro d’acquisition GenBank: BC153096
• Symbole du gène: LIFR
• Identification du gène (NCBI): 3977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIFR, also known as CD118, is a subunit of a receptor for leukemia inhibitory factor (LIF). LIF is a pleiotropic cytokine of the interleukin-6 family which affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of LIF. The high-affinity complex also binds a related cytokine, oncostatin M (PMID: 8999038). LIFR has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway (PMID: 23001183).

Publications

Species	Application	Title
mouse		Nature; Exercise plasma boosts memory and dampens brain inflammation via clusterin.; Authors - Zurine De Miguel
mouse	WB	Nat Commun; A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.; Authors - Fan Yao ; KO Validated
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
human	WB,IHC,IF	Hum Mol Genet; Mutations in the leukemia inhibitory factor receptor (LIFR) gene and Lifr deficiency cause urinary tract malformations.; Authors - Anne Kosfeld
human	WB	J Exp Clin Cancer Res; miR-589 promotes gastric cancer aggressiveness by a LIFR-PI3K/AKT-c-Jun regulatory feedback loop.; Authors - Feifei Zhang
mouse	WB	Biotechnol Bioeng; Enzyme-free cell detachment mediated by resonance vibration with temperature modulation.; Authors - Yuta Kurashina

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Clarisse (Verified Customer) (12-12-2022)

Low signal, regardless of the method of fixation.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human cerebral organoids

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