Anticorps Polyclonal de lapin anti-DNA ligase III/LIG3

• Phare
• Validé par KD/KO

DNA ligase III/LIG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 26583-1-AP

Synonymes

LIG3, ligase III, DNA, ATP dependent, LIG2, EC:6.5.1.1, DNA ligase III

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Galerie de données de validation

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  WB analysis using 26583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26583-1-AP (DNA ligase III/LIG3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26583-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26583-1-AP (DNA ligase III/LIG3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 26583-1-AP

  IP result of anti-DNA ligase III/LIG3 (IP:26583-1-AP, 4ug; Detection:26583-1-AP 1:300) with HEK-293T cells lysate 4000ug.

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  IHC staining of mouse testis using 26583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26583-1-AP (DNA ligase III/LIG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26583-1-AP (DNA ligase III/LIG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26583-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNA ligase III/LIG3 antibody (26583-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules PC-3
• Résultats positifs en IP: cellules HEK-293T
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

26583-1-AP cible DNA ligase III/LIG3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNA ligase III/LIG3 Protéine recombinante Ag24998
• Nom complet: ligase III, DNA, ATP-dependent
• Masse moléculaire calculée: 100-110 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC068005
• Symbole du gène: LIG3
• Identification du gène (NCBI): 3980
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA ligase III(DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

Publications

Species	Application	Title
human	IF	Brain; Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy.; Authors - Elena Bonora
pig	WB	Int J Biochem Cell Biol; Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.; Authors - Guoling Li
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
	WB	bioRxiv; D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability; Authors - Gergely Rona
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona
mouse	WB	J Alzheimers Dis Rep; Genome-Wide Mendelian Randomization Identifies Ferroptosis-Related Drug Targets for Alzheimer's Disease; Authors - Ying Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

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