Anticorps Polyclonal de lapin anti-LIG4

• Phare
• Validé par KD/KO

LIG4 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 12695-1-AP

Synonymes

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

---

Galerie de données de validation

• 

  WB analysis of mouse testis using 12695-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12695-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12695-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 12695-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human prostate cancer using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50.

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa, cellules HepG2, tissu hépatique de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IF: See 2 publications below

Informations sur le produit

12695-1-AP cible LIG4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIG4 Protéine recombinante Ag3385
• Nom complet: ligase IV, DNA, ATP-dependent
• Masse moléculaire calculée: 911 aa, 104 kDa
• Poids moléculaire observé: 100-104 kDa
• Numéro d’acquisition GenBank: BC037491
• Symbole du gène: LIG4
• Identification du gène (NCBI): 3981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

Publications

Species	Application	Title
human	WB	Cell Host Microbe; Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.; Authors - Paran Nir N ; KD Validated
human	WB	Nat Commun; Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.; Authors - Guijie Guo
human	IF	J Allergy Clin Immunol; XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency.; Authors - Chaowan Guo
mouse	WB	Nat Commun; Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy.; Authors - Ekta Khattar
human	WB	Nat Commun; Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.; Authors - Mengtan Xing
human	WB	Cancer Res; Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.; Authors - Qin Zhou