Anticorps Monoclonal anti-LIMK1

• Phare
• Validé par KD/KO

LIMK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2B11E9

N° de cat : 67974-1-Ig

Synonymes

LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 67974-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis using 67974-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HeLa using 67974-1-Ig

  WB result of LIMK1 antibody (67974-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMK1 transfected HeLa cells.

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  WB analysis of SH-SY5Y using 67974-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67974-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 67974-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67974-1-Ig (LIMK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 67974-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67974-1-Ig (LIMK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67974-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LIMK1 antibody (67974-1-Ig, Clone: 2B11E9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules MCF-7, cellules SH-SY5Y, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'estomac de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67974-1-Ig cible LIMK1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIMK1 Protéine recombinante Ag28862
• Nom complet: LIM domain kinase 1
• Masse moléculaire calculée: 647 aa, 73 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC152982
• Symbole du gène: LIMK1
• Identification du gène (NCBI): 3984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

Publications

Species	Application	Title
human	WB	Tissue Cell; B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway; Authors - Anjing Zhao
mouse	WB,IF	Zool Res; PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2-dependent control of the actin cytoskeleton, modulating emotional behavior; Authors - Laidong Yu

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