Anticorps Polyclonal de lapin anti-LIMS1

• Phare
• Validé par KD/KO

LIMS1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 20772-1-AP

Synonymes

LIMS1, PINCH, PINCH 1, PINCH1

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Galerie de données de validation

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  WB analysis of HeLa using 20772-1-AP

  WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells.

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  WB analysis of Caco-2 using 20772-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20772-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 20772-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 20772-1-AP

  IP Result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human oesophagus cancer using 20772-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus cancer using 20772-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20772-1-AP

  Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 20772-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LIMS1 antibody (20772-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HeLa, tissu pulmonaire de souris, tissu rénal humain
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

20772-1-AP cible LIMS1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIMS1 Protéine recombinante Ag14764
• Nom complet: LIM and senescent cell antigen-like domains 1
• Masse moléculaire calculée: 325 aa, 37 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC005341
• Symbole du gène: LIMS1
• Identification du gène (NCBI): 3987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo ; KO Validated
human	WB,IP	J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo ; KD Validated
human	WB	Theranostics; PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.; Authors - Xiaoxiao Wang
	WB	J Cell Sci; Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.; Authors - Clotilde Huet-Calderwood
human	WB	Amino Acids; PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.; Authors - Chunhong Cui ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yasmin (Verified Customer) (08-01-2019)

Excellent antibody for western blot. Works well at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000 for western blot
• Cell Tissue Type: HeLa, NIH3T3