Anticorps Polyclonal de lapin anti-LIN41

LIN41 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 55003-1-AP

Synonymes

TRIM71, RING-type E3 ubiquitin transferase TRIM71, Protein lin-41 homolog, Protein lin 41 homolog, LIN 41

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Galerie de données de validation

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  WB analysis of mouse kidney using 55003-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55003-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 55003-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 55003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules DU 145, cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

55003-1-AP cible LIN41 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tripartite motif-containing 71
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: NM_001039111
• Symbole du gène: LIN41
• Identification du gène (NCBI): 131405
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.

Publications

Species	Application	Title
human	WB	Cell Cycle; TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.; Authors - Daniel J Foster
human	WB	Cell Mol Gastroenterol Hepatol; HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation; Authors - Jingsheng Yuan
human,mouse	IHC	Mol Carcinog; Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC; Authors - Lilan Fan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This antibody produces very high background, using either milk or Licor blocking buffers, PVDF or nitrocellulose.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa, MT4 cells

LIN41 Antibody Western Blot validation (1:500 dilution) in HeLa, MT4 cells (Cat no:55003-1-AP)

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