Anticorps Monoclonal anti-LIPG

LIPG Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1B10B10

N° de cat : 67434-1-Ig

Synonymes

EDL, EL, Endothelial lipase, lipase, endothelial, LIPG, PRO719

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Galerie de données de validation

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  WB analysis using 67434-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human placenta using 67434-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67434-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HaCaT using 67434-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67434-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 67434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67434-1-Ig (LIPG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67434-1-Ig (LIPG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HaCaT, cellules HepG2, cellules HT-29, cellules MCF-7, cellules MDA-MB-468, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67434-1-Ig cible LIPG dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIPG Protéine recombinante Ag29482
• Nom complet: lipase, endothelial
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 and 42 kDa
• Numéro d’acquisition GenBank: BC060825
• Symbole du gène: LIPG
• Identification du gène (NCBI): 9388
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC,CoIP	Cancer Gene Ther; ZDHHC1 downregulates LIPG and inhibits colorectal cancer growth via IGF2BP1 Palmitoylation; Authors - Qun Zhang ; KD Validated

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