Anticorps Monoclonal anti-LIPH

LIPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1F5D10

N° de cat : 66303-1-Ig

Synonymes

AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B

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Galerie de données de validation

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  WB analysis of SW 1990 using 66303-1-Ig

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 66303-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66303-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 66303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human pancreas using 66303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human colon cancer using 66303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 66303-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 66303-1-Ig(LIPH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SW-1990, cellules BxPC-3, cellules COLO 320
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

66303-1-Ig cible LIPH dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIPH Protéine recombinante Ag24009
• Nom complet: lipase, member H
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC064941
• Symbole du gène: LIPH
• Identification du gène (NCBI): 200879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).

Publications

Species	Application	Title
human	IF	Genet Med; Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.; Authors - Liron Malki

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