Anticorps Polyclonal de lapin anti-LITAF

• Phare
• Validé par KD/KO

LITAF Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16797-1-AP

Synonymes

LITAF, LPS induced TNF alpha factor, p53 induced gene 7 protein, PIG7, SIMPLE, TP53I7

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Galerie de données de validation

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  WB analysis of HeLa using 16797-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16797-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16797-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16797-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16797-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16797-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16797-1-AP

  IP Result of anti-LITAF (IP:16797-1-AP, 4ug; Detection:16797-1-AP 1:600) with HeLa cells lysate 1520ug.

• 

  IHC staining of human spleen using 16797-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16797-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16797-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16797-1-AP (LITAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

16797-1-AP cible LITAF dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LITAF Protéine recombinante Ag10269
• Nom complet: lipopolysaccharide-induced TNF factor
• Masse moléculaire calculée: 161 aa, 17 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC008309
• Symbole du gène: LITAF
• Identification du gène (NCBI): 9516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lipopolysaccharide-induced TNF factor(LITAF) is a transcription factor that identified as a regulator of TNF-alpha gene expression. It may has a role in protein degradation pathways and in tumor necrosis factor alpha(TNF-alpha) gene expression. Also it may regulate the expression of the CCL2/MCP-1 chemokine through NFKB1.Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis

Publications

Species	Application	Title
human	WB	Cell Mol Neurobiol; LITAF Enhances Radiosensitivity of Human Glioma Cells via the FoxO1 Pathway.; Authors - Changlin Huang ; KD Validated
human	IHC	Nat Commun; Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis; Authors - Shu Zhang