Anticorps Polyclonal de lapin anti-LMBR1L

LMBR1L Polyclonal Antibody for FC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, FC,ELISA

Conjugaison

Non conjugué

N° de cat : 12154-1-AP

Synonymes

KIAA1174, LIMR, LMBR1L, Protein LMBR1L

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Galerie de données de validation

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  WB analysis of mouse testis using 12154-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12154-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12154-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 12154-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12154-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12154-1-AP

  IP Result of anti-LMBR1L (IP:12154-1-AP, 4ug; Detection:12154-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  FC experiment of A549 using 12154-1-AP

  1X10^6 A549 cells were stained with 0.2ug LMBR1L antibody (12154-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules A549, cellules HepG2, tissu hépatique de souris, tissu testiculaire de rat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en cytométrie: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12154-1-AP cible LMBR1L dans les applications de WB, IP, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LMBR1L Protéine recombinante Ag2787
• Nom complet: limb region 1 homolog (mouse)-like
• Masse moléculaire calculée: 489 aa, 55 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC015015
• Symbole du gène: LMBR1L
• Identification du gène (NCBI): 55716
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LMBR1L, also known as LIMR (lipocalin-1-interacting membrane receptor), interacts with lipocalin-1 (Lcn-1), a lipocalin member produced by a number of secretoric glands and tissues and known to bind a variety of lipophilic compounds. LMBR1L is a 489-amino acid protein with a predicted molecular mass of 55 kD and 9 putative transmembrane helices. LMBR1L has been shown to mediate cellular uptake of LMBR1L, thus it is characterized as a endocytic receptor (PMID: 11287427; 12591932). Alternatively spliced transcript variants encoding different isoforms have been described.