Anticorps Polyclonal de lapin anti-LMOD1

• Phare
• Validé par KD/KO

LMOD1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 15117-1-AP

Synonymes

1D, 64 kDa autoantigen 1D, 64 kDa autoantigen 1D3, 64 kDa autoantigen D1, 64kD, D1, Leiomodin 1, leiomodin 1 (smooth muscle), Leiomodin, muscle form, LMOD1, SM LMOD, Smooth muscle leiomodin

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Galerie de données de validation

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  WB analysis of HT-29 using 15117-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 15117-1-AP (LMOD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse bladder using 15117-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 15117-1-AP (LMOD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse uterus using 15117-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15117-1-AP (LMOD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15117-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15117-1-AP (LMOD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 15117-1-AP

  Immunofluorescent analysis of A375 cells, using LMOD1 antibody 15117-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu vésical de souris, cellules HT-29, tissu utérin de souris
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 9 publications below

Informations sur le produit

15117-1-AP cible LMOD1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LMOD1 Protéine recombinante Ag7176
• Nom complet: leiomodin 1 (smooth muscle)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 85 kDa, 64 kDa
• Numéro d’acquisition GenBank: BC001755
• Symbole du gène: LMOD1
• Identification du gène (NCBI): 25802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.

Publications

Species	Application	Title
human	IF,WB	Gastroenterology; The Long Non-coding RNA Carmn is a Critical Regulator of Gastrointestinal Smooth Muscle Contractile Function and Motility; Authors - Xiangqin He
human	IF	Nat Genet; Single-nucleus chromatin accessibility profiling highlights regulatory mechanisms of coronary artery disease risk.; Authors - Adam W Turner
mouse	IF	Sci Transl Med; Nodding syndrome may be an autoimmune reaction to the parasitic worm Onchocerca volvulus.; Authors - Tory P Johnson
mouse	IF	Genome Biol; Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression.; Authors - Pan Gao
mouse	WB,IF	Proc Natl Acad Sci U S A; Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice.; Authors - Danny Halim ; KO Validated
	WB,IF	PLoS Genet; Functional regulatory mechanism of smooth muscle cell-restricted LMOD1 coronary artery disease locus.; Authors - Vivek Nanda ; KD Validated