Anticorps Polyclonal de lapin anti-LMOD3

• Phare
• Validé par KD/KO

LMOD3 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 14948-1-AP

Synonymes

Leiomodin 3, leiomodin 3 (fetal), Leiomodin, fetal form, LMOD3

---

Galerie de données de validation

• 

  WB analysis using 14948-1-AP

  Mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 14948-1-AP (LMOD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 14948-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14948-1-AP (LMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14948-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14948-1-AP (LMOD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 14948-1-AP

  IP Result of anti-LMOD3 (IP:14948-1-AP, 4ug; Detection:14948-1-AP 1:700) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IHC staining of human heart using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de souris, tissu de muscle squelettique de rat
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

14948-1-AP cible LMOD3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LMOD3 Protéine recombinante Ag6758
• Nom complet: leiomodin 3 (fetal)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC039202
• Symbole du gène: LMOD3
• Identification du gène (NCBI): 56203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle.; Authors - Balázs Kiss
human, zebrafish	WB, IF	J Clin Invest; Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.; Authors - Michaela Yuen
mouse	WB	J Clin Invest; Severe myopathy in mice lacking the MEF2/SRF-dependent gene leiomodin-3.; Authors - Bercin K Cenik ; KO Validated
zebrafish	WB, IF	J Clin Invest; Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.; Authors - Michaela Yuen
human	WB	J Clin Invest; KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy.; Authors - Ankit Garg
mouse	WB	Proc Natl Acad Sci U S A; HSPB7 is indispensable for heart development by modulating actin filament assembly.; Authors - Tongbin Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tongbin (Verified Customer) (08-25-2020)

This antibody is very specific. I had a single Lmod3 band at around 70kD. Western blot results of this antibody had been published in our PNAS paper (PMID: 29078393)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

LMOD3 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:14948-1-AP)