Anticorps Polyclonal de lapin anti-LNPEP

LNPEP Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 55154-1-AP

Synonymes

CAP, Cystinyl aminopeptidase, IRAP, Leucyl cystinyl aminopeptidase, leucyl/cystinyl aminopeptidase, LNPEP, OTase, Oxytocinase, P LAP, PLAP, VP165

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Galerie de données de validation

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  WB analysis of human brain using 55154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 55154-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55154-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 55154-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules COLO 320, tissu cardiaque humain
• Résultats positifs en IHC: tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55154-1-AP cible LNPEP dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: leucyl/cystinyl aminopeptidase
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 117-125 kDa
• Numéro d’acquisition GenBank: NM_005575
• Symbole du gène: LNPEP
• Identification du gène (NCBI): 4012
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.