Anticorps Polyclonal de lapin anti-Loricrin

Loricrin Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(29)

N° de cat : 55439-1-AP

Synonymes

LOR, LRN

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Galerie de données de validation

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  IHC staining of mouse skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human paracancerous tissue of skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of skin slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human paracancerous tissue of skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of skin slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 55439-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Loricrin antibody (55439-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en IHC: tissu cutané de souris, human paracancerous tissue of skin; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 10 publications below
• IF: See 11 publications below

Informations sur le produit

55439-1-AP cible Loricrin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: loricrin
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: NM_000427
• Symbole du gène: Loricrin
• Identification du gène (NCBI): 4014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.

Publications

Species	Application	Title
mouse	WB,IF	Cell Death Differ; ENKD1 promotes epidermal stratification by regulating spindle orientation in basal keratinocytes.; Authors - Tao Zhong
mouse	WB	EMBO Rep; Regulation of epidermal differentiation through KDF1-mediated deubiquitination of IKKα.; Authors - Yuanyuan Li
mouse,human	WB,IF	Br J Pharmacol; FGF21 promotes migration and differentiation of epidermal cells during wound healing via SIRT1 dependent autophagy.; Authors - Xixi Chen
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
mouse	WB,IF	J Ethnopharmacol; Topical application of Artemisia annua L. essential oil ameliorates 2,4-dintrochlorobenzene-induced atopic dermatitis in mice; Authors - Zhiya Huang
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan

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