Anticorps Polyclonal de lapin anti-LOXL2

LOXL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 15232-1-AP

Synonymes

EC:1.4.3.13, LOR2, Lysyl oxidase-related protein WS9-14, WS9 14, WS914

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Galerie de données de validation

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  WB analysis using 15232-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15232-1-AP (LOXL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15232-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15232-1-AP (LOXL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15232-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15232-1-AP (LOXL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15232-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15232-1-AP (LOXL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15232-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LOXL2 antibody (15232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15232-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LOXL2 antibody (15232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MDA-MB-453s
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

15232-1-AP cible LOXL2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LOXL2 Protéine recombinante Ag7083
• Nom complet: lysyl oxidase-like 2
• Masse moléculaire calculée: 774 aa, 87 kDa
• Poids moléculaire observé: 68 kDa. 100 kDa
• Numéro d’acquisition GenBank: BC000594
• Symbole du gène: LOXL2
• Identification du gène (NCBI): 4017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression.

Publications

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
mouse	WB	J Ethnopharmacol; Escin Ia Ameliorates DSS-Induced Chronic Colitis in Mice by Inhibiting Inflammation and Oxidative Stress via the LOXL2/MMP-9 Pathway; Authors - Jing Yan
human	WB	J Cancer; Dihydroartemisinin Potentiates VEGFR-TKIs Antitumorigenic Effect on Osteosarcoma by Regulating Loxl2/VEGFA Expression and Lipid Metabolism Pathway; Authors - Xiaomin Ding

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