Anticorps Monoclonal anti-LOXL2

LOXL2 Monoclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1H7F1

Avis (1)

N° de cat : 67139-1-Ig

Synonymes

LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14

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Galerie de données de validation

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  WB analysis of A549 using 67139-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67139-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 67139-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67139-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 67139-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 67139-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67139-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67139-1-Ig (LOXL2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A375, tissu cardiaque humain, tissu placentaire humain
• Résultats positifs en IHC: tissu de côlon de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

67139-1-Ig cible LOXL2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LOXL2 Protéine recombinante Ag28566
• Nom complet: lysyl oxidase-like 2
• Masse moléculaire calculée: 774 aa, 87 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC000594
• Symbole du gène: LOXL2
• Identification du gène (NCBI): 4017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).

Publications

Species	Application	Title
human		J Clin Invest; LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis; Authors - Yingzhi Shen
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
mouse	WB,IF	Int J Biol Macromol; Depletion of LOXL2 improves respiratory capacity: From air-breathing fish to mammal under hypoxia.; Authors - Bing Sun
human	WB	Oncogene; METTL1/WDR4-mediated tRNA m7G modification and mRNA translation control promote oncogenesis and doxorubicin resistance; Authors - Zhaoyu Wang
human	IF	Neuron; Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier; Authors - Yuan Xie
human	WB,IHC	Neoplasia; β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis; Authors - Yijia Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (10-04-2021)

The calculated MW is 87 kd. This antibody recognized major bands at 65, and 60 kd.

• Applications: Western Blot

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