Anticorps Polyclonal de lapin anti-EDG7-Specific

EDG7-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 19509-1-AP

Synonymes

Edg 7, EDG7, GPCR, HOFNH30, LP A3, LPA 3, LPA receptor 3, LPA3, LPAR3, RP4 678I3

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Galerie de données de validation

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  WB analysis of PC-3 using 19509-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19509-1-AP (EDG7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 19509-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19509-1-AP (EDG7-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19509-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19509-1-AP (EDG7-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19509-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19509-1-AP (EDG7-Specific antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 19509-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19509-1-AP (EDG7-Specific antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse heart using 19509-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19509-1-AP (EDG7-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, tissu pancréatique de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

19509-1-AP cible EDG7-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lysophosphatidic acid receptor 3
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: NM_012152
• Symbole du gène: LPAR3
• Identification du gène (NCBI): 23566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPAR3, also named as EDG7 and LPA3, belongs to the G-protein coupled receptor 1 family. LPAR3 is a receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. LPAR3 may play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins. The antibody is specific to LPAR3.

Publications

Species	Application	Title
human	IHC	Comput Struct Biotechnol J; Interrelations of Sphingolipid and Lysophosphatidate Signaling with Immune System in Ovarian Cancer.; Authors - Anastasia Meshcheryakova
mouse	WB	Pediatr Res; Bronchopulmonary dysplasia demonstrates dysregulated autotaxin/lysophosphatidic acid signaling in a neonatal mouse model; Authors - Alison W Ha
human	IF	Nat Commun; Time-series single-cell transcriptomic profiling of luteal-phase endometrium uncovers dynamic characteristics and its dysregulation in recurrent implantation failures; Authors - Dandan Cao

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