Anticorps Polyclonal de lapin anti-LPCAT2

LPCAT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (2)

N° de cat : 15082-1-AP

Synonymes

1-acylglycerol-3-phosphate O-acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase, 1-AGP acyltransferase 11, 1-AGPAT 11, 1-alkenylglycerophosphocholine O-acyltransferase

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Galerie de données de validation

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  WB analysis using 15082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15082-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of LPS treated HEK-293 using 15082-1-AP

  LPS treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15082-1-AP

  IP result of anti-LPCAT2 (IP:15082-1-AP, 4ug; Detection:15082-1-AP 1:5000) with HeLa cells lysate 1280 ug.

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  IHC staining of mouse brain using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat thyroid gland using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat thyroid gland using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 15082-1-AP

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15082-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LPCAT2 antibody (15082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293 traitées au LPS, cellules HeLa, cellules Jurkat, cellules RAW 264.7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: rat thyroid gland tissue, tissu cérébral de souris, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15082-1-AP cible LPCAT2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LPCAT2 Protéine recombinante Ag7156
• Nom complet: lysophosphatidylcholine acyltransferase 2
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC002472
• Symbole du gène: LPCAT2
• Identification du gène (NCBI): 54947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Antioxidants (Basel); Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis; Authors - Melanie Merkel

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melanie (Verified Customer) (04-22-2022)

LPCAT2 antibody was diluted 1:1500 in 5% milk in TBST and applied to protein samples from Hek293T cells. The bands were very clear. The antibody could have been diluted even more.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: Hek293T cells

FH

Christian (Verified Customer) (12-17-2018)

10 μg protein per lane run on 10% Tris-Glycine gel. Primary incubation 1:400 overnight @ 4°C. Secondary antibody and biotinylated marker were supplied from a different vendor and used and used at optimized dilutions.LPCAT2 siRNA was used to check antibody specificity.The antibody performed excellently, will try at a dilution of 1:800 next time.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: RAW264.7

LPCAT2 Antibody Western Blot, validation (1:400 dilution) in RAW264.7 (Cat no:15082-1-AP)

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