Anticorps Monoclonal anti-LPCAT3

• Phare
• Validé par KD/KO

LPCAT3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(27)

CloneNo.

1C1G3

N° de cat : 67882-1-Ig

Synonymes

1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphoethanolamine O-acyltransferase, 1-acylglycerophosphoserine O-acyltransferase, 1C1G3, C3F

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Galerie de données de validation

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  WB analysis of HepG2 using 67882-1-Ig

  WB result of LPCAT3 antibody (67882-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPCAT3 transfected HepG2 cells.

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  WB analysis of pig stomach using 67882-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 67882-1-Ig (LPCAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de porc, cellules HepG2
• Résultats positifs en IHC: tissu d'estomac humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 27 publications below
• IF: See 2 publications below

Informations sur le produit

67882-1-Ig cible LPCAT3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LPCAT3 Protéine recombinante Ag6472
• Nom complet: lysophosphatidylcholine acyltransferase 3
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC065194
• Symbole du gène: LPCAT3
• Identification du gène (NCBI): 10162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysophosphatidylcholine acyltransferases (LPCATs) are among the lysophopholipid acyltransferases (LPLATs) that play an important role in lipid metabolism and homeostasis by regulating the abundance of different phosphatidylcholine (PC) species. Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is a member of the LPCAT family that primarily regulates the levels of arachidonic PC species. LPCAT3 is abundantly expressed in the testes, kidneys, and metabolic tissues, including the liver, intestine, and adipose tissues. LPCAT3 is involved in the occurrence and development of atherosclerosis, intestinal tumors, and nonalcoholic steatohepatitis (NASH)(PMID: 35711841).

Publications

Species	Application	Title
human	WB	J Immunother Cancer; Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors; Authors - Qian Tao ; KD Validated
	WB	Free Radic Biol Med; Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury; Authors - Wei Deng
	WB	Phytomedicine; Rosmarinic acid liposomes suppress ferroptosis in ischemic brain via inhibition of TfR1 in BMECs; Authors - Cui-Ling Jia
	WB,IF	Phytomedicine; MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice; Authors - Jia Shi
human	WB	J Cell Biol; The deubiquitinase ZRANB1 is an E3 ubiquitin ligase for SLC7A11 and regulates ferroptotic resistance; Authors - Shan Huang
	WB	Inflammation; Investigation of Metabolic and Inflammatory Disorder in the Aging FGF21 Knockout Mouse; Authors - Lu-Qiong Cai

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