Anticorps Monoclonal anti-LPCAT3

LPCAT3 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

1C1G3

N° de cat : 67882-1-Ig

Synonymes

C3F, LPCAT, LPCAT3, LPLAT 5, LPSAT, Lyso PC acyltransferase, Lyso PC acyltransferase 3, Lyso PS acyltransferase, MBOAT5, nessy, OACT5

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Galerie de données de validation

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  WB analysis of mouse stomach using 67882-1-Ig

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 67882-1-Ig (LPCAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig stomach using 67882-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 67882-1-Ig (LPCAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 67882-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de souris, tissu d'estomac de porc
• Résultats positifs en IHC: tissu d'estomac humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

67882-1-Ig cible LPCAT3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LPCAT3 Protéine recombinante Ag6472
• Nom complet: lysophosphatidylcholine acyltransferase 3
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC065194
• Symbole du gène: LPCAT3
• Identification du gène (NCBI): 10162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysophosphatidylcholine acyltransferases (LPCATs) are among the lysophopholipid acyltransferases (LPLATs) that play an important role in lipid metabolism and homeostasis by regulating the abundance of different phosphatidylcholine (PC) species. Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is a member of the LPCAT family that primarily regulates the levels of arachidonic PC species. LPCAT3 is abundantly expressed in the testes, kidneys and metabolic tissues, including liver, intestine and adipose tissues. LPCAT3 is involved in the occurrence and development of atherosclerosis, intestinal tumors, and nonalcoholic steatohepatitis (NASH)(PMID: 35711841).

Publications

Species	Application	Title
mouse	WB	Environ Toxicol; Di-(2-ethylhexyl) phthalate induces ferroptosis in prepubertal mouse testes via the lipid metabolism pathway; Authors - Xia Wang
human	WB	J Exp Clin Cancer Res; Lipid reprogramming induced by the TFEB-ERRα axis enhanced membrane fluidity to promote EC progression.; Authors - Xiaodan Mao
human	WB	Ecotoxicol Environ Saf; Cadmium perturbed lipid profile and induced liver dysfunction in mice through phosphatidylcholine remodeling and promoting arachidonic acid synthesis and metabolism; Authors - Jie Gu
rat	WB	Sci Total Environ; Insight into the effect of a heavy metal mixture on neurological damage in rats through combined serum metabolomic and brain proteomic analyses; Authors - Jie Xie
human	WB	Redox Biol; Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis; Authors - Xue Yang