Anticorps Polyclonal de lapin anti-LPCAT4

• Phare
• Validé par KD/KO

LPCAT4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 17905-1-AP

Synonymes

1-AGP acyltransferase 7, 1-acylglycerophosphoserine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerol-3-phosphate O-acyltransferase 7, 1 AGPAT 7

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Galerie de données de validation

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  WB analysis using 17905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17905-1-AP (LPCAT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17905-1-AP

  WB result of LPCAT4 antibody (17905-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPCAT4 transfected HeLa cells.

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  IHC staining of human intrahepatic cholangiocarcinoma using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

17905-1-AP cible LPCAT4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LPCAT4 Protéine recombinante Ag12281
• Nom complet: lysophosphatidylcholine acyltransferase 4
• Masse moléculaire calculée: 524 aa, 57 kDa
• Poids moléculaire observé: 50-57 kDa
• Numéro d’acquisition GenBank: BC092463
• Symbole du gène: LPCAT4
• Identification du gène (NCBI): 254531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPCAT4, also known as lysophosphatidylcholine acyltransferase 4, is an enzyme that plays a crucial role in lipid metabolism and membrane remodeling It is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family and is involved in the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction. The protein is predicted to be located in the membrane and is classified as a metabolic protein and enzyme. It is involved in biological processes such as lipid biosynthesis, lipid metabolism, phospholipid biosynthesis, and phospholipid metabolism. .

Publications

Species	Application	Title
human	WB	J Hepatol; Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.; Authors - Morita Yoshifumi Y
mouse	WB	Free Radic Biol Med; Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury; Authors - Wei Deng
mouse	WB	Neurochem Res; iPLA2β Knockout Mouse, a Genetic Model for Progressive Human Motor Disorders, Develops Age-Related Neuropathology.; Authors - Helene Blanchard
human	WB	FASEB J; Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas' disease progression; Authors - Chunhua Liu
human	WB	Front Biosci (Landmark Ed); Upregulation of LPGAT1 Enhances Lung Adenocarcinoma Proliferation; Authors - Huiyuan Gong
mouse	WB	FASEB J; Overexpression of lysophospholipid acyltransferase, LPLAT10/LPCAT4/LPEAT2, in the mouse liver increases glucose-stimulated insulin secretion; Authors - Kahori Shimizu

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