Anticorps Polyclonal de lapin anti-LPHN3

LPHN3 Polyclonal Antibody for FC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, FC,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 20045-1-AP

Synonymes

CIRL 3, CIRL3, KIAA0768, latrophilin 3, LEC3, Lectomedin 3, LPHN3

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Galerie de données de validation

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  WB analysis of mouse brain using 20045-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20045-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20045-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 20045-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 20045-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20045-1-AP

  IP Result of anti-LPHN3 (IP:20045-1-AP, 4ug; Detection:20045-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  FC experiment of HepG2 using 20045-1-AP

  1X10^6 HepG2 cells were stained with .2ug LPHN3 antibody (20045-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules COLO 320, cellules HepG2, tissu cérébral de rat, tissu de côlon de souris, tissu hépatique humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

20045-1-AP cible LPHN3 dans les applications de WB, IP, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: latrophilin 3
• Masse moléculaire calculée: 162 kDa
• Poids moléculaire observé: 139 kDa
• Numéro d’acquisition GenBank: NM_015236
• Symbole du gène: LPHN3
• Identification du gène (NCBI): 23284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.

Publications

Species	Application	Title
human	WB	World J Biol Psychiatry; Expression of the adult ADHD-associated gene ADGRL3 is dysregulated by risk variants and environmental risk factors.; Authors - Rhiannon V McNeill