Anticorps Polyclonal de lapin anti-LPP

LPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 25045-1-AP

Synonymes

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

---

Galerie de données de validation

• 

  WB analysis using 25045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25045-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25045-1-AP

  IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of human small intestine using 25045-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 25045-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 25045-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 25045-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 25045-1-AP

  Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules NIH/3T3, cœur de rat, cœur de souris, côlon de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

25045-1-AP cible LPP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LPP Protéine recombinante Ag19205
• Nom complet: LIM domain containing preferred translocation partner in lipoma
• Masse moléculaire calculée: 612 aa, 66 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC130584
• Symbole du gène: LPP
• Identification du gène (NCBI): 4026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).

Publications

Species	Application	Title
human	WB,IHC,IF	Cell Death Dis; UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer; Authors - Kanghua Xiao

{{ptg:RelatedPrimaryAntibodies}}