Anticorps Polyclonal de lapin anti-LRAT

LRAT Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 12815-1-AP

Synonymes

LRAT

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Galerie de données de validation

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  WB analysis of mouse testis using 12815-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12815-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

12815-1-AP cible LRAT dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRAT Protéine recombinante Ag3556
• Nom complet: lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
• Masse moléculaire calculée: 230 aa, 26 kDa
• Poids moléculaire observé: 26 kDa, 50-54 kDa
• Numéro d’acquisition GenBank: BC031053
• Symbole du gène: LRAT
• Identification du gène (NCBI): 9227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

Publications

Species	Application	Title
rat	WB	iScience; Control by the brain of vitamin A homeostasis; Authors - Peter I Imoesi
rat	WB	Am J Pathol; Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.; Authors - Volha V Malechka
mouse	WB	J Food Biochem; Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.; Authors - Wei Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Peter (Verified Customer) (12-03-2018)

The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Liver Tissue

LRAT Antibody Western Blot, validation (1:500 dilution) in Rat Liver Tissue (Cat no:12815-1-AP)