Anticorps Polyclonal de lapin anti-LRMP

LRMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 19498-1-AP

Synonymes

Inositol 1,4,5-triphosphate receptor associated 2, IRAG2, JAW1, Lymphoid-restricted membrane protein, Processed inositol 1,4,5-triphosphate receptor associated 2

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Galerie de données de validation

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  WB analysis of Raji using 19498-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19498-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 19498-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 19498-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of Jurkat using 19498-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LRMP antibody (19498-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of Jurkat using 19498-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug LRMP Polyclonal antibody (19498-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Jurkat
• Résultats positifs en IHC: tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Jurkat,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19498-1-AP cible LRMP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lymphoid-restricted membrane protein
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_006152
• Symbole du gène: LRMP
• Identification du gène (NCBI): 4033
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRMP, also named as JAW1, is a lymphoid restricted integral membrane protein that is localised to the cytoplasmic face of the endoplasmic reticulum.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (10-06-2024)

We tried flow cytometry (FC) with both human and mouse cells and it did now work for either. However, to be fair, the data sheet does not report FC as one of the tested applications and it also says that it does not work in mouse, so it is expected. If Proteintech could develop an anti-mouse LRMP that would be amazing, we haven't found any that works.

• Applications: Flow Cytometry
• Cell Tissue Type: Human and mouse

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