Anticorps Polyclonal de lapin anti-LRP2-Specific

• Phare
• Validé par KD/KO

LRP2-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (2)

N° de cat : 19700-1-AP

Synonymes

LRP2, LRP 2, Low-density lipoprotein receptor-related protein 2, gp330, Glycoprotein 330

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Galerie de données de validation

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  WB analysis using 19700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 19700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 19700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris,
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Informations sur le produit

19700-1-AP cible LRP2-Specific dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: low density lipoprotein-related protein 2
• Masse moléculaire calculée: 522 kDa
• Poids moléculaire observé: 522 kDa
• Numéro d’acquisition GenBank: NM_004525
• Symbole du gène: LRP2
• Identification du gène (NCBI): 4036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS).

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits; Authors - Mengmeng Wang
mouse	IF	ACS Appl Mater Interfaces; Renal-Targeted Drug Delivery by Chitosan Oligosaccharide Micelles with HSA-Enriched Protein Corona for the Treatment of Ischemia/Reperfusion-Induced Acute Kidney Injury; Authors - Simin Nan
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
human	WB,IHC	Nephrol Dial Transplant; Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.; Authors - Hiwa Fatah
mouse	WB	Development; Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.; Authors - Congshan Sun
mouse	IHC,IF	Metabolism; Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.; Authors - Hitomi Otomo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval on paraffin embedded mouse kidney sections. Developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue

LRP2-Specific Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse Kidney Tissue (Cat no:19700-1-AP)

FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval and developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue

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