Anticorps Polyclonal de lapin anti-LRP2-Specific

• Phare
• Validé par KD/KO

LRP2-Specific Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 19700-1-AP

Synonymes

DBS, Fx1a, Glycoprotein 330, gp330, LRP 2, LRP2, LRP2-Specific, Megalin

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Galerie de données de validation

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  WB analysis of HeLa using 19700-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19700-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19700-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19700-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 19700-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug LRP2-Specific antibody (19700-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cérébral humain, tissu placentaire humain
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

19700-1-AP cible LRP2-Specific dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: low density lipoprotein-related protein 2
• Masse moléculaire calculée: 522 kDa
• Poids moléculaire observé: 280 kDa
• Numéro d’acquisition GenBank: NM_004525
• Symbole du gène: LRP2
• Identification du gène (NCBI): 4036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS). This antibody is specific to LRP2 and detects endogenous LRP2 with an apparent molecular weight 280 kDa, which is smaller than the predicted molecular weight (522 kDa) and probably represent a proteolytic fragment of the large glycoprotein (PMID: 16638803; 8483924).

Publications

Species	Application	Title
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
human	WB,IHC	Nephrol Dial Transplant; Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.; Authors - Hiwa Fatah
mouse	WB	Development; Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.; Authors - Congshan Sun
mouse	IHC,IF	Metabolism; Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.; Authors - Hitomi Otomo
mouse	WB	Mol Ther Nucleic Acids; Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway.; Authors - Bohong Cen
human	WB	Stem Cell Res; Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system.; Authors - Jie You ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval on paraffin embedded mouse kidney sections. Developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue

LRP2-Specific Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse Kidney Tissue (Cat no:19700-1-AP)

FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval and developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue

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