Anticorps Polyclonal de lapin anti-LRRC32

LRRC32 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 26021-1-AP

Synonymes

D11S833E, GARP, Garpin, LRRC32

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Galerie de données de validation

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  WB analysis of HUVEC using 26021-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 26021-1-AP (LRRC32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HUVEC,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

26021-1-AP cible LRRC32 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRRC32 Protéine recombinante Ag23423
• Nom complet: leucine rich repeat containing 32
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC070079
• Symbole du gène: LRRC32
• Identification du gène (NCBI): 2615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats (PMID: 8180135). LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells (PMID: 27054568). It has been demonstrated as a Treg-specific activation marker (PMID: 20137067). LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor (PMID: 19651619).

Publications

Species	Application	Title
mouse	WB	iScience; GRP94 promotes anoikis resistance and peritoneal metastasis through YAP/TEAD1 pathway in gastric cancer; Authors - Qimeng Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Martina (Verified Customer) (07-09-2021)

Antibody worked well for WB and IHC, specificity confirmed by knockdown and over-extpression

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:500 WB 1:5000 IHC
• Cell Tissue Type: Human Skin

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