Anticorps Polyclonal de lapin anti-LSM5

LSM5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 10700-1-AP

Synonymes

YER146W, U6 snRNA-associated Sm-like protein LSm5

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Galerie de données de validation

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  WB analysis of HeLa using 10700-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis using 10700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 10700-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10700-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10700-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10700-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10700-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10700-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10700-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10700-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10700-1-AP (LSM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HL-60, tissu cérébral humain, tissu ovarien de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10700-1-AP cible LSM5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LSM5 Protéine recombinante Ag1145
• Nom complet: LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC005938
• Symbole du gène: LSM5
• Identification du gène (NCBI): 23658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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