Anticorps Polyclonal de lapin anti-LSS

LSS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 13715-1-AP

Synonymes

hOSC, Lanosterol synthase, LSS, OSC

---

Galerie de données de validation

• 

  WB analysis using 13715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13715-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13715-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13715-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13715-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13715-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13715-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13715-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13715-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13715-1-AP

  IP Result of anti-LSS (IP:13715-1-AP, 3ug; Detection:13715-1-AP 1:500) with HepG2 cells lysate 2900ug.

• 

  IHC staining of human liver using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13715-1-AP

  Immunofluorescent analysis of HepG2 cells using 13715-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 13715-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LSS antibody (13715-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: HT-1376 cells, cellules HeLa, cellules HepG2, cellules PC-3, cellules SH-SY5Y, tissu cérébral humain, tissu hépatique de souris, tissu pancréatique de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13715-1-AP cible LSS dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LSS Protéine recombinante Ag4655
• Nom complet: lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
• Masse moléculaire calculée: 732 aa, 83 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC035638
• Symbole du gène: LSS
• Identification du gène (NCBI): 4047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.

Publications

Species	Application	Title
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	WB	J Invest Dermatol; Bi-allelic Variants in LSS Cause Palmoplantar Keratoderma-Congenital Alopecia Syndrome Type 2.; Authors - Fang Yang
human	WB,IHC	PLoS Genet; Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.; Authors - Yoichi Wada
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
human	WB, IHC	Phytomedicine; Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.; Authors - Xing Yue Xu
human	WB	J Biol Chem; The antiviral enzyme viperin inhibits cholesterol biosynthesis.; Authors - Timothy J Grunkemeyer