Anticorps Polyclonal de lapin anti-LTA4H

LTA4H Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13662-1-AP

Synonymes

Leukotriene A 4 hydrolase, Leukotriene A(4) hydrolase, leukotriene A4 hydrolase, LTA 4 hydrolase, LTA4, LTA4H

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Galerie de données de validation

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  WB analysis of HEK-293 using 13662-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13662-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13662-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13662-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13662-1-AP

  IP Result of anti-LTA4H (IP:13662-1-AP, 4ug; Detection:13662-1-AP 1:500) with HEK-293 cells lysate 1120ug.

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  IHC staining of human skin using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cutané humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13662-1-AP cible LTA4H dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LTA4H Protéine recombinante Ag4568
• Nom complet: leukotriene A4 hydrolase
• Masse moléculaire calculée: 611 aa, 69 kDa
• Poids moléculaire observé: 57-70 kDa
• Numéro d’acquisition GenBank: BC032528
• Symbole du gène: LTA4H
• Identification du gène (NCBI): 4048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LTA4H(Leukotriene A-4 hydrolase), also named as LTA4, is essential for the conversion of LTA4 to LTB4, an inflammatory lipid mediator(PMID:10601658). LTA4H also possesses aminopeptidase activity that it can degrade PGP, which is a biomarker for chronic obstructive pulmonary disease and is implicated in neutrophil persistence in the lung(PMID:20813919). This protein has 4 isoforms produced by alternative splicing with the MW of 69, 59, 57, 67 kDa.

Publications

Species	Application	Title
human	WB	Proteomics; Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.; Authors - Wei Gao
mouse	IF	JHEP Rep; Time-resolved single-cell transcriptomics reveals the landscape and dynamics of hepatic cells in sepsis-induced acute liver dysfunction; Authors - Gan Chen