Anticorps Polyclonal de lapin anti-LTBP1

• Phare
• Validé par KD/KO

LTBP1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 26855-1-AP

Synonymes

LTBP 1, LTBP1

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Galerie de données de validation

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  WB analysis of human plasma using 26855-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 26855-1-AP (LTBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26855-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26855-1-AP (LTBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

26855-1-AP cible LTBP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LTBP1 Protéine recombinante Ag25392
• Nom complet: latent transforming growth factor beta binding protein 1
• Masse moléculaire calculée: 1721 aa, 187 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC130289
• Symbole du gène: LTBP1
• Identification du gène (NCBI): 4052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Latent TGFβ binding protein 1 (LTBP1) is a large extracellular protein which belongs to the family of latent TGF-beta binding proteins (LTBPs). LTBP1 is essential for TGF-beta folding, secretion, matrix localization and activation. LTBP1 has also been demonstrated to interact with a number of insoluble extracellular matrix components, such as fibrillin. We got 150-240 kDa in western blotting due to protein glycosylation of LTBP1 (PMID: 18086923, PMID: 9923648 ).

Publications

Species	Application	Title
human	IF	Cell Rep; Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis; Authors - Jose Verdezoto Mosquera
human	WB,IHC	J Transl Med; LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation.; Authors - Rui Cai ; KD Validated
human	WB	Cell Death Discov; ERRFI1 induces apoptosis of hepatocellular carcinoma cells in response to tryptophan deficiency.; Authors - Mingqing Cui
human,mouse	IHC	medRxiv; Secreted protein profiling of human aortic smooth muscle cells identifies vascular disease associations; Authors - Rédouane Aherrahrou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (10-05-2022)

The Ab was used at 1:1000 dilution in NFDM overnight 4 degrees and detected a band close to 220 kD in 20ug cell lysate. Also tested for for positive IF staining at 1:250 dilution in 2% BSA overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Bone Marrow MSCs

LTBP1 Antibody Western Blot validation (1:1000 dilution) in Bone Marrow MSCs (Cat no:26855-1-AP)