Anticorps Polyclonal de lapin anti-LTF

• Phare
• Validé par KD/KO

LTF Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10933-1-AP

Synonymes

GIG12, HLF2, Lactoferrin, lactotransferrin, LF, LTF, Talalactoferrin

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Galerie de données de validation

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  WB analysis using 10933-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10933-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human pancreas using 10933-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10933-1-AP

  IP Result of anti-Lactoferrin (IP:10933-1-AP, 4ug; Detection:10933-1-AP 1:600) with HeLa cells lysate 4000ug.

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  IHC staining of human liver using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human prostate hyperplasia using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 40x lens).

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Applications testées

• Résultats positifs en WB: salive humain, cellules HEK-293, tissu pancréatique humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below

Informations sur le produit

10933-1-AP cible LTF dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LTF Protéine recombinante Ag1379
• Nom complet: lactotransferrin
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 30,51,80 kDa
• Numéro d’acquisition GenBank: BC015822
• Symbole du gène: LTF
• Identification du gène (NCBI): 4057
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).

Publications

Species	Application	Title
human	IHC	Sci Adv; Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.; Authors - Takafumi Kato
human	WB,IHC	Cancer Cell Int; Identification and validation of an immune-related prognostic signature and key gene in papillary thyroid carcinoma.; Authors - Rujia Qin
human	WB	Biochem Biophys Res Commun; NEDD4L-mediated LTF protein degradation limits ferroptosis.; Authors - Yuan Wang ; KD Validated
human	IHC	Int Med Case Rep J; Collagen fiber changes related to keratoconus with secondary corneal amyloidosis.; Authors - Kaoru Araki-Sasaki
mouse	WB	Bioact Mater; Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment; Authors - Qiong Huang