Anticorps Polyclonal de lapin anti-LYN

LYN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 18135-1-AP

Synonymes

EC:2.7.10.2, JTK8, Lck/Yes-related novel protein tyrosine kinase, Tyrosine protein kinase Lyn, Tyrosine-protein kinase Lyn

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Galerie de données de validation

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  WB analysis of HeLa using 18135-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 18135-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 18135-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18135-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18135-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18135-1-AP

  IP result of anti-LYN (IP:18135-1-AP, 4ug; Detection:18135-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 18135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 18135-1-AP

  Immunofluorescent analysis of HeLa cells using 18135-1-AP (LYN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18135-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LYN Polyclonal antibody (18135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HL-60, cellules Jurkat, cellules K-562, cellules L02
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

18135-1-AP cible LYN dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LYN Protéine recombinante Ag12723
• Nom complet: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Masse moléculaire calculée: 512 aa, 59 kDa
• Poids moléculaire observé: 53-56 kDa, 66-70 kDa
• Numéro d’acquisition GenBank: BC075002
• Symbole du gène: LYN
• Identification du gène (NCBI): 4067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Publications

Species	Application	Title
human	WB,IF	J Invest Dermatol; Loss of LYN is frequent in targeted-therapy resistant melanoma cells and favors metastatic properties; Authors - Céline Gaudel ; KD Validated
human	IHC	Front Cell Dev Biol; Immune Characteristics of LYN in Tumor Microenvironment of Gliomas.; Authors - Chonghua Jiang

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