Anticorps Polyclonal de lapin anti-LYPLAL1

• Phare
• Validé par KD/KO

LYPLAL1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 16146-1-AP

Synonymes

EC:3.1.2.22, KIAA1238, lysophospholipase like 1, Lysophospholipase-like protein 1

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Galerie de données de validation

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  WB analysis using 16146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16146-1-AP (LYPLAL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16146-1-AP

  WB result of LYPLAL1 antibody (16146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LYPLAL1 transfected HeLa cells.

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  IHC staining of human liver cancer using 16146-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16146-1-AP (LYPLAL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16146-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16146-1-AP (LYPLAL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16146-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16146-1-AP (LYPLAL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16146-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16146-1-AP (LYPLAL1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16146-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16146-1-AP (LYPLAL1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

16146-1-AP cible LYPLAL1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LYPLAL1 Protéine recombinante Ag9271
• Nom complet: lysophospholipase-like 1
• Masse moléculaire calculée: 237 aa, 26 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC016711
• Symbole du gène: LYPLAL1
• Identification du gène (NCBI): 127018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysophospholipase-like 1 protein, encoded by LYPLAL1, a 26 kDa cytosol protein which belongs to a subclass of lysophospholipase family. Human genome-wide association studies link variants of the gene encoding this enzyme to fat distribution, waist-to-hip ratio and non-alcoholic fatty liver disease. LYPLAL1 reported to be up-regulated in subcutaneous adipose tissue of obese individuals.(PMID: 25958046, PMID: 27391855, PMID: 21674055)

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Discovery of small-molecule enzyme activators by activity-based protein profiling.; Authors - Bernard P Kok
mouse	WB	Dis Model Mech; Lyplal1 is dispensable for normal fat deposition in mice.; Authors - Rachel A Watson ; KO Validated
mouse,human	WB,IHC	Mol Cell; Targeting LYPLAL1-mediated cGAS depalmitoylation enhances the response to anti-tumor immunotherapy; Authors - Yizeng Fan
mouse	WB	J Mol Endocrinol; Knockout of murine Lyplal1 confers sex-specific protection against diet-induced obesity; Authors - Rishel B Vohnoutka

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bernard (Verified Customer) (11-11-2019)

Works perfectly for overexpressed protein and endogenous protein in 293T cells

• Applications: Western Blot,
• Cell Tissue Type: 293T

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