• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Lin28A-specific

Lin28A-specific Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 16177-1-AP

Synonymes

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Lin28A-specific, Protein lin 28 homolog A, ZCCHC1



Applications testées

Résultats positifs en WBcellules SCEh, cellule NCCIT, tissu cardiaque humain, tissu cérébral humain, tissu hépatique de souris
Résultats positifs en IHCtissu d'intestin grêle humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochimie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

16177-1-AP cible Lin28A-specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet lin-28 homolog (C. elegans)
Masse moléculaire calculée 23 kDa
Poids moléculaire observé 28 kDa
Numéro d’acquisition GenBankNM_024674
Symbole du gène LIN28
Identification du gène (NCBI) 79727
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human LIN28.This antibody recognize Lin28A specifically.

Protocole

Product Specific Protocols
WB protocol for Lin28A-specific antibody 16177-1-APDownload protocol
IHC protocol for Lin28A-specific antibody 16177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

LIN28A enhances regenerative capacity of human somatic tissue stem cells via metabolic and mitochondrial reprogramming.

Authors - Kelvin Pieknell
humanWB

Cancer Res

Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.

Authors - Domenico Albino
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
  • KD Validated
humanWB

EMBO Rep

The human FOXM1 homolog promotes basal progenitor cell proliferation and cortical folding in mouse.

Authors - Wenwen Wang
humanWB

Cell Death Dis

Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A.

Authors - Qin He
humanWB

J Virol

Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.

Authors - Ali Naushad N

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carolina (Verified Customer) (09-26-2019)

Antibody was more specific than the previous Lin28A antibody.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:20
  • Cell Tissue Type: Human Testis
FH

KUNHUA (Verified Customer) (02-01-2019)

Got a very strong band at 25-37kd with 1:1000 concentration.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human erythroid progenitor