Anticorps Monoclonal anti-Ly-6G

Ly-6G Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

1A8

N° de cat : PE-65078

Synonymes

Ly6g, Ly-6G.1, Ly 6G, 1A8

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using PE-65078

  1x10^6 mouse bone marrow cells were surface stained with 0.2 ug PE Anti-Mouse Ly-6G (1A8) (PE-65078, Clone:1A8) or 0.2 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65078 cible Ly-6G dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée cellulaire EL-4J transfectée par Ly-6G
• Nom complet: lymphocyte antigen 6 complex, locus G
• Numéro d’acquisition GenBank: X70920
• Symbole du gène: Ly-6G
• Identification du gène (NCBI): 546644
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).

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