Anticorps Monoclonal anti-Lysozyme

Lysozyme Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

2G5A8

N° de cat : 66456-1-Ig

Synonymes

LYZ, 1,4-beta-N-acetylmuramidase C, 2G5A8, EC:3.2.1.17, Lysozyme C

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Galerie de données de validation

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  WB analysis of human spleen using 66456-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 66456-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 66456-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66456-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 66456-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66456-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique humain, cellules RAW 264.7, tissu splénique de porc, tissu splénique de rat
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66456-1-Ig cible Lysozyme dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lysozyme Protéine recombinante Ag6917
• Nom complet: lysozyme (renal amyloidosis)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC004147
• Symbole du gène: Lysozyme
• Identification du gène (NCBI): 4069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Publications

Species	Application	Title
mouse	WB	Cell Rep; Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia; Authors - Xin Xu
rat	IHC	J Pharmacol Sci; Oxymatrine alleviates NSAID-associated small bowel mucosal injury by regulating MIP-1/CCR1 signalling and gut microbiota; Authors - Ming Chen
human	IHC	Clin Transl Med; Single-cell transcriptome analysis reveals evolving tumour microenvironment induced by immunochemotherapy in nasopharyngeal carcinoma; Authors - Yaofei Jiang
human	WB,IF	Biomater Res; A New Light-Sensor System Affecting Cancer Cell Fate; Authors - Silvia Buonvino
mouse	WB	Res Sq; Sex-dependent effect of GPR109A gene deletion in myeloid cells on bone development in mice; Authors - Perry C Caviness

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