Anticorps Monoclonal anti-M-CSF

M-CSF Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

3G12E6

N° de cat : 65283-1-Ig

Synonymes

3G12E6, CSF 1, CSF1, CSF-1, Lanimostim

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Galerie de données de validation

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  FC experiment of human PBMCs using 65283-1-Ig

  1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 405 Anti-Human CD11b then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65283-1-Ig.

• 

  FC experiment of human PBMCs using 65283-1-Ig

  1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65283-1-Ig.

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Applications testées

• Résultats positifs en FC (Intra): LPS, TNF-α and Brefeldin A treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65283-1-Ig cible M-CSF dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: M-CSF Protéine recombinante HZ-1192
• Nom complet: colony stimulating factor 1 (macrophage)
• Masse moléculaire calculée: 60 kDa
• Numéro d’acquisition GenBank: BC021117
• Symbole du gène: M-CSF
• Identification du gène (NCBI): 1435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

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