Validation Data Gallery
Technical Specifications
| GeneID | 1435 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.7-4.0 ng/mL |
| Specific Activity | minimally 4.00 x 107 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P09603 |
| Molecular Mass | 19 and 23 kDa reduced, 35 and 40 kDa non-reduced, homodimer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)
Synonyms
CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF
Publications
| Species | Title |
|---|---|
Signal Transduct Target Ther Targeting GPR34 in damage-associated macrophages enhances anti-tumor immunity and the efficacy of Surufatinib in pancreatic cancer. | |
Cell Metab A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis. | |
Cell Metab Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain | |
Cell Metab Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK | |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Federico (Verified Customer) (12-22-2025) | It works perfectly. I use it in culture media to generate macrophages or microglia from hiPSCs.
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FH Lucy (Verified Customer) (11-07-2025) | I used this product to differentiate monocyte-derived macrophages and they looked beautiful! Really pleased with the performance of this product.
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FH Alicia (Verified Customer) (10-14-2025) | I am using MCSF for my human iPSC derived microglia.
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FH Ariane (Verified Customer) (03-28-2025) | Used it to promote macrophage growth from bone marrow derived cells; used it at a conc. of 10ng/mL seems to work
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FH amandine (Verified Customer) (01-13-2025) | we are still doing comparizon between our previous M-CSF (from another company) , for now the MCSF from proteintech works better but we still need to perfom some experiment for control (growing of BMDMs). if the results are good, we will buy your Proteintech MSCF, because it is anyway less expensive than the other one we use
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FH Romane (Verified Customer) (12-06-2024) | Works great for differentiation of human iPSCs-derived macrophages
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