Anticorps Polyclonal de lapin anti-MAGED1

MAGED1 Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11539-1-AP

Synonymes

DLXIN 1, MAGE D1 antigen, MAGE tumor antigen CCF, MAGED1, melanoma antigen family D, 1, Melanoma associated antigen D1, NRAGE, PP2250

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 11539-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 11539-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11539-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11539-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11539-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11539-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11539-1-AP (MAGED1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 11539-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11539-1-AP (MAGED1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of HeLa using 11539-1-AP

  1X10^6 HeLa cells were stained with 0.2ug MAGED1 antibody (11539-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HeLa apoptosées, tissu cérébral humain
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11539-1-AP cible MAGED1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGED1 Protéine recombinante Ag2109
• Nom complet: melanoma antigen family D, 1
• Masse moléculaire calculée: 86 kDa; 92 kDa
• Poids moléculaire observé: 66 kDa, 92-95 kDa
• Numéro d’acquisition GenBank: BC014070
• Symbole du gène: MAGED1
• Identification du gène (NCBI): 9500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527).

Publications

Species	Application	Title
rat	WB,IHC,IF,IP	Sci Rep; Dynamic expression of Mage-D1 in rat dental germs and potential role in mineralization of ectomesenchymal stem cells; Authors - Meng Li