Anticorps Polyclonal de lapin anti-MAGED2

• Phare
• Validé par KD/KO

MAGED2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15252-1-AP

Synonymes

11B6, HCA10, JCL 1, MAGE D2, MAGE D2 antigen, MAGED, MAGED2, melanoma antigen family D, 2, Melanoma associated antigen D2

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Galerie de données de validation

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  WB analysis using 15252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15252-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15252-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15252-1-AP

  IP Result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug.

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  IHC staining of human breast cancer using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15252-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15252-1-AP cible MAGED2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGED2 Protéine recombinante Ag6828
• Nom complet: melanoma antigen family D, 2
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC000304
• Symbole du gène: MAGED2
• Identification du gène (NCBI): 10916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Nephrol Dial Transplant; MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells.; Authors - Lara Valiño-Rivas
mouse	WB	J Neurosci; MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation.; Authors - Mouri Akihiro A
human	WB	Biochem Pharmacol; Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.; Authors - Charlotte Trussart ; KD Validated
human	WB	RNA; An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.; Authors - Ewan K S McRae
human	WB,IF	Biochim Biophys Acta; Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.; Authors - Céline Pirlot ; KD Validated
human	WB,IP	mBio; SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense; Authors - Xiaohui Ju ; KD Validated