Anticorps Polyclonal de lapin anti-MAML1

MAML1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, CoIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 55493-1-AP

Synonymes

KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1

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Galerie de données de validation

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  WB analysis using 55493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

55493-1-AP cible MAML1 dans les applications de WB, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mastermind-like 1 (Drosophila)
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 108-120 kDa
• Numéro d’acquisition GenBank: NM_014757
• Symbole du gène: MAML1
• Identification du gène (NCBI): 9794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.

Publications

Species	Application	Title
human	WB,CoIP	Int J Biol Sci; Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.; Authors - Yanzhu Shi
human	WB	Discov Oncol; CSF2 promotes chemoresistance in colorectal cancer by regulating Notch pathway; Authors - Hairong Zhou

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