Anticorps Polyclonal de lapin anti-LC3

LC3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(73)

N° de cat : 12135-1-AP

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 12135-1-AP

  Chloroquine treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12135-1-AP (LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12135-1-AP

  Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 12135-1-AP (LC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12135-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12135-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12135-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12135-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12135-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 12135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12135-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 12135-1-AP

  Immunofluorescent analysis of starvation treated HEK-293 cells using 12135-1-AP (LC3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with ethanol at -20 °C. 20 mM chloroquine was used to block the autophagy flux.

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Applications testées

• Résultats positifs en WB: cellules HEK-293 traitées à la chloroquine, cellules HeLa traitées à la chloroquine
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293 traitées par déprivation,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 60 publications below
• IHC: See 7 publications below
• IF: See 22 publications below

Informations sur le produit

12135-1-AP cible LC3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LC3 Protéine recombinante Ag2781
• Nom complet: microtubule-associated protein 1 light chain 3 alpha
• Masse moléculaire calculée: 121 aa, 14 kDa
• Numéro d’acquisition GenBank: BC015810
• Symbole du gène: LC3
• Identification du gène (NCBI): 84557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I (18-20 kDa) and LC3-II (14-16 kDa).

Publications

Species	Application	Title
mouse	IF,WB	Autophagy; Analysis of ATG4C function in vivo; Authors - Isaac Tamargo-Gómez
mouse	WB	J Clin Invest; Autophagy is essential for mouse sense of balance.; Authors - Mari?o Guillermo G
mouse	WB	Autophagy; Assessing autophagy in the context of photodynamic therapy.; Authors - Reiners John J JJ Jr
mouse	WB	Cell Death Differ; ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.; Authors - Isaac Tamargo-Gómez
human	WB	EMBO J; Unsaturated fatty acids induce non-canonical autophagy.; Authors - Mireia Niso-Santano
human	WB	Oxid Med Cell Longev; Enhanced p62-NRF2 Feedback Loop due to Impaired Autophagic Flux Contributes to Arsenic-Induced Malignant Transformation of Human Keratinocytes.; Authors - Xiafang Wu

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