Anticorps Monoclonal anti-MAP2

MAP2 Monoclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

CloneNo.

1C3E6

Avis (2)

N° de cat : 67015-1-Ig

Synonymes

MAP 2, MAP2, MAP2A, MAP2B, MAP2C

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Galerie de données de validation

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  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 67015-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67015-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67015-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Neuro-2a using 67015-1-Ig

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of SH-SY5Y using 67015-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de rat, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en cytométrie: cellules SH-SY5Y, cellules Neuro-2a

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 15 publications below
• IP: See 1 publications below

Informations sur le produit

67015-1-Ig cible MAP2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MAP2 Protéine recombinante Ag11349
• Nom complet: microtubule-associated protein 2
• Masse moléculaire calculée: 200 kDa
• Numéro d’acquisition GenBank: BC038857
• Symbole du gène: MAP2
• Identification du gène (NCBI): 4133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

Publications

Species	Application	Title
mouse	IF	Cell; Revisiting astrocyte to neuron conversion with lineage tracing in vivo.; Authors - Lei-Lei Wang
mouse	IF	Cell Stem Cell; In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.; Authors - Wenjiao Tai ; KO Validated
rat	IF	Small; Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.; Authors - Xiheng Lu
rat	IF	Cell Death Dis; ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis; Authors - Yaxuan Zhang
mouse	WB,IF	Cell Death Dis; Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes; Authors - Yuan Yao
mouse	IF	Acta Neuropathol Commun; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.; Authors - Tuancheng Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

• Applications: Immunofluorescence
• Cell Tissue Type: Cortical organoids

FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the MAP2 antibody at the 1/1000 dilution was used overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human primary cortical cells

MAP2 Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical cells (Cat no:67015-1-Ig)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C