Anticorps Polyclonal de lapin anti-MEK1/2

MEK1/2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

47

N° de cat : 11049-1-AP

Synonymes

ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 1/2, MEK 2, MEK1/2, MEK2, MKK2, PRKMK2

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Galerie de données de validation

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  WB analysis of HL-60 using 11049-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11049-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11049-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11049-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 11049-1-AP

  IP Result of anti-MEK2 (IP:11049-1-AP, 4ug; Detection:11049-1-AP 1:1000) with HL-60 cells lysate 1280ug.

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  IHC staining of mouse brain using 11049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 11049-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (11049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 11049-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP (MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HL-60, cellules Jurkat, cellules SH-SY5Y, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 46 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11049-1-AP cible MEK1/2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEK1/2 Protéine recombinante Ag1481
• Nom complet: mitogen-activated protein kinase kinase 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44-47 kDa
• Numéro d’acquisition GenBank: BC018645
• Symbole du gène: MAP2K2
• Identification du gène (NCBI): 5605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.

Publications

Species	Application	Title
mouse	WB	Nat Commun; ZFP281-BRCA2 prevents R-loop accumulation during DNA replication.; Authors - Yan Wang
mouse	WB	ACS Appl Mater Interfaces; Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.; Authors - Chaoqun Li
mouse,human	WB	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou
human	WB	Cell Death Dis; The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.; Authors - Wenjuan Li
human	WB	Int J Mol Sci; Cytostatic Activity of Sanguinarine and a Cyanide Derivative in Human Erythroleukemia Cells Is Mediated by Suppression of c-MET/MAPK Signaling; Authors - Xinglian Xu