Anticorps Recombinant de lapin anti-MAP2K3

• Phare
• Validé par KD/KO

MAP2K3 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3O10

N° de cat : 80137-1-RR

Synonymes

MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2

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Galerie de données de validation

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  WB analysis using 80137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 80137-1-RR

  WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells.

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  WB analysis using 80137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 80137-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 80137-1-RR

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80137-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 80137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue)

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Applications testées

• Résultats positifs en WB: cellules C6, cellules A431, cellules C2C12, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HT-1080, cellules Jurkat, cellules NIH/3T3, tissu de poisson-zèbre
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

80137-1-RR cible MAP2K3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 3
• Masse moléculaire calculée: 347 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC032478
• Symbole du gène: MAP2K3
• Identification du gène (NCBI): 5606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP2K3, also named MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

Publications

Species	Application	Title
mouse	WB	Acta Pharm Sin B; Reversing metabolic reprogramming by CPT1 inhibition with etomoxir promotes cardiomyocyte proliferation and heart regeneration via DUSP1 ADP-ribosylation-mediated p38 MAPK phosphorylation; Authors - Luxun Tang

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