Anticorps Polyclonal de lapin anti-MLK3

• Phare
• Validé par KD/KO

MLK3 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11996-1-AP

Synonymes

MAP3K11, Mixed lineage kinase 3, MLK 3, MLK3, PTK1, SPRK

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Galerie de données de validation

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  WB analysis using 11996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11996-1-AP (MLK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11996-1-AP

  WB result of MLK3 antibody (11996-1-AP, 1:500) with si-Control and si-MLK3 transfected HEK293 cells.

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 11996-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLK3 antibody (11996-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11996-1-AP

  Immunofluorescent analysis of Hela cells, using MAP3K11 antibody 11996-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules C6, cellules HEK-293, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11996-1-AP cible MLK3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLK3 Protéine recombinante Ag2636
• Nom complet: mitogen-activated protein kinase kinase kinase 11
• Masse moléculaire calculée: 847 aa, 93 kDa
• Poids moléculaire observé: 93 and 65 kDa
• Numéro d’acquisition GenBank: BC011263
• Symbole du gène: MAP3K11
• Identification du gène (NCBI): 4296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).

Publications

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy.; Authors - Jie Huang
human	WB	J Virol; High-throughput screening identifies mixed lineage kinase 3 as a key host regulatory factor in Zika virus infection.; Authors - Hua Xu
mouse	WB	J Ethnopharmacol; Xinyang Tablet inhibits MLK3-mediated pyroptosis to attenuate inflammation and cardiac dysfunction in pressure overload.; Authors - Junyan Wang
human	IP, WB, IF	Front Physiol; Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.; Authors - Changjiang Ying
mouse	WB	J Mol Histol; Silence of MLK3 alleviates lipopolysaccharide-induced lung epithelial cell injury via inhibiting p53-mediated ferroptosis.; Authors - Xiangjun Chen
mouse	WB	Cell Death Dis; Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload.; Authors - Junyan Wang