Anticorps Polyclonal de lapin anti-TAB2

• Phare
• Validé par KD/KO

TAB2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 14410-1-AP

Synonymes

KIAA0733, MAP3K7IP2, TAB 2, TAB2, TAK1 binding protein 2

---

Galerie de données de validation

• 

  WB analysis using 14410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14410-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 14410-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14410-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14410-1-AP

  IP Result of anti-TAB2 (IP:14410-1-AP, 4ug; Detection:14410-1-AP 1:600) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human malignant melanoma using 14410-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 14410-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 14410-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAP3K7IP2 antibody 14410-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 14410-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug TAB2 antibody (14410-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules L02, cellules C6, cellules HepG2, cellules Raji, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de mélanome malin humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14410-1-AP cible TAB2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAB2 Protéine recombinante Ag5742
• Nom complet: mitogen-activated protein kinase kinase kinase 7 interacting protein 2
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC035910
• Symbole du gène: MAP3K7IP2
• Identification du gène (NCBI): 23118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tab2 has a role in the inflammatory signal transduction pathway and is involved in the reversal of NCoR-dependent and NFkB- or APP-mediated gene repression and androgen antagonist response. Besides, it is identified as a cofactor for ERBB4 to assemble NCoR corepressor complex in response to neuregulin [PMID:16469706]. It also functions as an adaptor that links TAK1 and TRAF6 in response to interleukin-1β (IL-1β) or tumour necrosis factor-α (TNF-α), and thereby mediates TAK1 activation [PMID:11460167].

Publications

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nat Commun; Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.; Authors - Chunjie Sheng
human	WB	Cell Biol Toxicol; microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.; Authors - Qiuyue Li ; KD Validated
mouse	WB	Front Immunol; MicroRNA 27a-3p Regulates Antimicrobial Responses of Murine Macrophages Infected byMycobacterium aviumsubspeciesparatuberculosisby Targeting Interleukin-10 and TGF-β-Activated Protein Kinase 1 Binding Protein 2.; Authors - Tariq Hussain
human	WB	J Neuroinflammation; miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses.; Authors - Bo Yang
human	WB	Front Immunol; Hepatitis B Surface Antigen Suppresses the Activation of Nuclear Factor Kappa B Pathway via Interaction With the TAK1-TAB2 Complex.; Authors - Feiyan Deng