Anticorps Polyclonal de lapin anti-MAP4K4

• Phare
• Validé par KD/KO

MAP4K4 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 55247-1-AP

Synonymes

EC:2.7.11.1, FLH21957, HGK, HPK/GCK like kinase HGK, HPK/GCK-like kinase HGK

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Galerie de données de validation

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  WB analysis of Jurkat using 55247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55247-1-AP

  WB result of MAP4K4 antibody (55247-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP4K4 transfected Jurkat cells.

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  WB analysis using 55247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 55247-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 55247-1-AP

  IP result of anti-MAP4K4 (IP:55247-1-AP, 4ug; Detection:55247-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: HK-2 cells, cellules Jurkat, cellules PC-3
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu hépatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

55247-1-AP cible MAP4K4 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase kinase kinase 4
• Masse moléculaire calculée: 151 kDa
• Poids moléculaire observé: 130-160 kDa
• Numéro d’acquisition GenBank: NM_004834
• Symbole du gène: MAP4K4
• Identification du gène (NCBI): 9448
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Artesunate improves glucose and lipid metabolism in db/db mice by regulating the metabolic profile and the MAPK/PI3K/Akt signalling pathway; Authors - Lulu Chen
human	WB	Anal Chem; Parallel-Reaction-Monitoring-Based Proteome-Wide Profiling of Differential Kinase Protein Expression during Prostate Cancer Metastasis in Vitro.; Authors - Weili Miao
human	WB	EBioMedicine; Targeting miR-30d reverses pathological cardiac hypertrophy.; Authors - Jin Li
mouse	WB	J Proteome Res; Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.; Authors - Jingzi Zhang
mouse	WB,IF	Neural Regen Res; MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.; Authors - Zheng Zou ; KD Validated
human	IHC	PeerJ; Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma.; Authors - Wanzhen Li

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