Anticorps Polyclonal de lapin anti-SIN1/MAPKAP1

SIN1/MAPKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (2)

N° de cat : 15463-1-AP

Synonymes

MAPKAP1, MIP1, Mitogen-activated protein kinase 2-associated protein 1, SAPK interacting protein 1, SIN1

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Galerie de données de validation

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  WB analysis of mouse kidney using 15463-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15463-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 15463-1-AP

  IP result of anti-SIN1 (IP:15463-1-AP, 4ug; Detection:15463-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15463-1-AP (SIN1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SIN1 antibody (15463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

15463-1-AP cible SIN1/MAPKAP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIN1/MAPKAP1 Protéine recombinante Ag7753
• Nom complet: mitogen-activated protein kinase associated protein 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC002326
• Symbole du gène: MAPKAP1
• Identification du gène (NCBI): 79109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitogen-activated protein kinase 2-associated protein 1 (MAPKAP1) is also named as MIP1 and SIN1. SIN1/MAPKAP1 is a major partner of mTORC2, acting as a scaffold and responsible for the substrate specificity of the mTOR catalytic subunit (PMID: 37877351). Besides as a component of MTORC2, MAPKAP1 also functions as MEKK2 and Ras-interacting protein and involves in regulating MEKK2 activation and RAS signaling (PMID: 31773361).

Publications

Species	Application	Title
mouse	IF	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
human	WB	EMBO Rep; Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling; Authors - Misha Kalarikkal ; KD Validated
mouse	WB	Cell Rep; Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.; Authors - Fangfang Sun
mouse	WB	bioRxiv; Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter; Authors - Marco Di Gioia
mouse	IF	bioRxiv; Ibudilast Protects Retinal Bipolar Cells from Excitotoxic Retinal Damage and Activates the mTOR Pathway; Authors - Sumaya Hamadmad
human	WB,IHC	Cell Signal; Overexpression of microRNA-611 inhibits TGF-β-induced epithelial-mesenchymal transition and migration in lung cancer cells through MAPKAP1; Authors - Bin Ke

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shile (Verified Customer) (07-24-2025)

The SIN1 rabbit polyclonal antibody works well in our WB (1:500).

• Applications: Western Blot
• Primary Antibody Dilution: WB 1:500
• Cell Tissue Type: Human Rh30 cancer cell lysates

FH

Shile (Verified Customer) (01-31-2025)

15463-1-AP SIN1 Rabbit PolyAb was working well in our Western blotting (1:500).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 for WB
• Cell Tissue Type: 293T cell lysates

SIN1/MAPKAP1 Antibody Western Blot validation (1:500 for WB dilution) in 293T cell lysates (Cat no:15463-1-AP)

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