Anticorps Polyclonal de lapin anti-EB3

EB3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 23974-1-AP

Synonymes

EB3, End binding protein 3, MAPRE3

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Galerie de données de validation

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  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23974-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 23974-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EB3 antibody (23974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

23974-1-AP cible EB3 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EB3 Protéine recombinante Ag21148
• Nom complet: microtubule-associated protein, RP/EB family, member 3
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 30-36 kDa
• Numéro d’acquisition GenBank: BC011557
• Symbole du gène: EB3
• Identification du gène (NCBI): 22924
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	WB	Cell Tissue Res; Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress; Authors - Valentina Grande
rat	IF	Neural Regen Res; The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons; Authors - Shi-Yan Sun

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